px330 (Addgene inc)
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Addgene inc
px330
Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pm41928626-196-3-4
Average 96 stars, based on 2984 article reviews
Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pm41928626-196-3-4
Average 96 stars, based on 2984 article reviews
px330 - by Bioz Stars,
2026-09
96/100 stars
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Related Articles
CRISPR:Article Title: Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition. Article Snippet: Short hairpin RNA (shRNA) sequences targeting UHRF1 were cloned into pLKO.1 (Addgene, 10878). .. Single guide RNAs (sgRNAs) were designed with the Benchling CRISPR Guide RNA Design tool ( https://www.benchling.com/crispr ) and subcloned into shRNA:Article Title: Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition. Article Snippet: Short hairpin RNA (shRNA) sequences targeting UHRF1 were cloned into pLKO.1 (Addgene, 10878). .. Single guide RNAs (sgRNAs) were designed with the Benchling CRISPR Guide RNA Design tool ( https://www.benchling.com/crispr ) and subcloned into Clone Assay:Article Title: IAP Antagonists Selectively Eliminate Therapy‐Induced Senescent Cancer Cells via TNFα ‐Independent Apoptosis Article Snippet: .. The annealed oligonucleotide containing TNFα sgRNA was cloned into the Bbs I site of Plasmid Preparation:Article Title: IAP Antagonists Selectively Eliminate Therapy‐Induced Senescent Cancer Cells via TNFα ‐Independent Apoptosis Article Snippet: .. The annealed oligonucleotide containing TNFα sgRNA was cloned into the Bbs I site of Article Title: Methods of gene therapy Article Snippet: .. Oligonucleotides encoding this sequence were annealed and ligated into Generated:Article Title: IAP Antagonists Selectively Eliminate Therapy‐Induced Senescent Cancer Cells via TNFα ‐Independent Apoptosis Article Snippet: .. The annealed oligonucleotide containing TNFα sgRNA was cloned into the Bbs I site of Article Title: Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing Article Snippet: Plasmids encoding PEmax, MLH1dn, or iCre were linearized as templates for IVT using the MESSAGE mMACHINE T7 ULTRA Kit (Thermo Fisher Scientific). .. Templates for epegRNA and sgRNA were generated by PCR amplification of Article Title: Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing Article Snippet: Plasmids encoding PEmax, MLH1dn, or iCre were linearized as templates for IVT using the MESSAGE mMACHINE T7 ULTRA Kit (Thermo Fisher Scientific). .. Templates for epegRNA and sgRNA were generated by PCR amplification of Polymerase Chain Reaction:Article Title: Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing Article Snippet: Plasmids encoding PEmax, MLH1dn, or iCre were linearized as templates for IVT using the MESSAGE mMACHINE T7 ULTRA Kit (Thermo Fisher Scientific). .. Templates for epegRNA and sgRNA were generated by PCR amplification of Article Title: A programmable platform enabling targeted chromosome substitution and cross-species stability profiling. Article Snippet: The T7-Cas9 32 PCR product was purified using Gel Extraction Kit (Omega) and used as the template for in vitro 33 transcription (IVT) of Cas9 mRNA using the mMESSAGE mMACHINE T7 kit (Life Technologies). .. 34 To prepare sgRNAs, the T7 promoter sequence was added to the sgRNA template by PCR 35 amplification of Article Title: Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing Article Snippet: Plasmids encoding PEmax, MLH1dn, or iCre were linearized as templates for IVT using the MESSAGE mMACHINE T7 ULTRA Kit (Thermo Fisher Scientific). .. Templates for epegRNA and sgRNA were generated by PCR amplification of Amplification:Article Title: Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing Article Snippet: Plasmids encoding PEmax, MLH1dn, or iCre were linearized as templates for IVT using the MESSAGE mMACHINE T7 ULTRA Kit (Thermo Fisher Scientific). .. Templates for epegRNA and sgRNA were generated by PCR amplification of Article Title: A programmable platform enabling targeted chromosome substitution and cross-species stability profiling. Article Snippet: The T7-Cas9 32 PCR product was purified using Gel Extraction Kit (Omega) and used as the template for in vitro 33 transcription (IVT) of Cas9 mRNA using the mMESSAGE mMACHINE T7 kit (Life Technologies). .. 34 To prepare sgRNAs, the T7 promoter sequence was added to the sgRNA template by PCR 35 amplification of Article Title: Prime Editing Exhibits Limited Genome-Wide Off-Target Effects in Cellular and Embryonic Gene Editing Article Snippet: Plasmids encoding PEmax, MLH1dn, or iCre were linearized as templates for IVT using the MESSAGE mMACHINE T7 ULTRA Kit (Thermo Fisher Scientific). .. Templates for epegRNA and sgRNA were generated by PCR amplification of Sequencing:Article Title: Methods of gene therapy Article Snippet: .. Oligonucleotides encoding this sequence were annealed and ligated into Article Title: A programmable platform enabling targeted chromosome substitution and cross-species stability profiling. Article Snippet: The T7-Cas9 32 PCR product was purified using Gel Extraction Kit (Omega) and used as the template for in vitro 33 transcription (IVT) of Cas9 mRNA using the mMESSAGE mMACHINE T7 kit (Life Technologies). .. 34 To prepare sgRNAs, the T7 promoter sequence was added to the sgRNA template by PCR 35 amplification of RNA Expression:Article Title: Methods of gene therapy Article Snippet: .. Oligonucleotides encoding this sequence were annealed and ligated into |